Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity
نویسندگان
چکیده
منابع مشابه
A Multiple Reaction Monitoring (MRM) Method to Detect Bcr-Abl Kinase Activity in CML Using a Peptide Biosensor
The protein kinase Bcr-Abl plays a major role in the pathogenesis of chronic myelogenous leukemia (CML), and is the target of the breakthrough drug imatinib (Gleevec™). While most patients respond well to imatinib, approximately 30% never achieve remission or develop resistance within 1-5 years of starting imatinib treatment. Evidence from clinical studies suggests that achieving at least 50% i...
متن کاملMiniaturized fiber-optic biosensor to monitor asparagine in clinical samples
Asparagine biosensor was constructed by co-immobilized anti-leukemic enzyme (L-asparaginase) and absorptive pH sensitive indicator on to plastic chips of 5mm through hydro sol-gel approach. Asparagine is used as a biomarker for detection of acute lymphoblastic leukemia. In this research work asparagine biosensor was developed focusing on L-asparaginase activity as bioassay principle. L-asparagi...
متن کاملReusable amperometric biosensor for measuring protein tyrosine kinase activity.
This work presents a simple, low-cost and reusable label-free method for detecting protein tyrosine kinase activity using a tyrosinase-based amperometric biosensor (tyrosine kinase biosensor). This method is based on the observation that phosphorylation can block the tyrosinase-catalyzed oxidation of tyrosine or tyrosyl residue in peptides. Therefore, the activity of p60c-src protein tyrosine k...
متن کاملA reliable self-assembled peptide based electrochemical biosensor for detection of caspase 3 activity and apoptosis
A sensitive electrochemical self-assembled peptide based biosensor was developed for detection of caspase 3 activity and apoptosis using the Asp-Glu-Val-Asp (DEVD) modified peptide and horseradish peroxidase (HRP) as cleaving and electron transfer (current amplifier) agents, respectively. Streptavidin coated magnetic beads (MB) were used to increase the loading efficiency of HRP on the DEVD mod...
متن کاملPhotonic Crystal Kinase Biosensor
We have developed a novel biosensor for kinases that is based on a kinase-responsive polymer hydrogel, which enables label-free screening of kinase activity via changes in optical properties. The hydrogel is specifically designed to swell reversibly upon phosphorylation of a target peptide, triggering a change in optical diffraction from a crystalline colloidal array of particles impregnated in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical Communications
سال: 2017
ISSN: 1359-7345,1364-548X
DOI: 10.1039/c6cc09948c